ebrB [2019-07-10 11:56:40]

multidrug efflux transporter

locus: BSU17290

pI: 6.93

mw: 12.19 kDa

protein length: 117 aa

gene length: 354 bp

function: multidrug resistance

product: multidrug efflux transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.14|Multidrug exporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,864,691 1,865,044

The protein

Protein family

• paired small multidrug resistance protein family ([SW|PSMR family]) [Pubmed|17942072]

Paralogous protein(s)

• [protein|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|EbrA]

Structure

• [PDB|3B5D] (EmrE from E. coli, 39% identity) [pubmed|18024586]

[SW|Localization]

• cell membrane [Pubmed|17417881]

Expression and Regulation

Operons

• genes: [gene|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|ebrA]-[gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B077 (ebrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1076 NBRP B. subtilis, Japan]
• BKE17290 ([gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCCTCTCATCTTCATCA, downstream forward: _UP4_GCCTGTGAGTGACCGTCTGT
• BKK17290 ([gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCCTCTCATCTTCATCA, downstream forward: _UP4_GCCTGTGAGTGACCGTCTGT

References

Reviews

• 17942072

Original publications

• 17417881,18024586